Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
![Q. Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β β-galactoside production and the recombinant plasmid is inserted in an E.coli strain, 1. it will lead to the lysis of host cells. 2. it will be able to produce a novel protein with dual abilities. 3. it will not be able to confer ampicillin resistance to the host cell. 4. the transformed cells will have the ability to resist ampicillin as well as produce β β-galactoside. [NEET 2021]](https://blogger.googleusercontent.com/img/a/AVvXsEiQawFhvDFtuyhRbUutREtdhleBYevoInb0-keHq6I3oLNC1zXu1i9rgcmI9i9fMnDbisrml1vRuffPgR5oYiOh1AJbEDGhrxFPw1VtlTGQZXiBSUb6YJBLb9UDpUvnQWKGZ4LHiiJqJ6HkSIWvITivaVxmGMKxL6A94wKqnvgT6ydna_IJuJvT-hm2jA=w74-h74-p-k-no-nu)
![During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out: (1) RNA (2) DNA (3) Histones (4) Polysaccharides [NEET 2021]](https://blogger.googleusercontent.com/img/a/AVvXsEiUYtv_y2xPUP53GLq0VpYFM4NA2eJWEr-a_XOTkH3064uNvC1nyJ5KMLV0zloDDbjzv2Bnob_Encj0NUKb8do1rcXQ8hnMcpzXxPZidL6mh8pmx_fOJ81lNA5oWARg39HF1fSYxbz_--vASKH90Rzr99-zH5JwrKIDwW3Faxpei8IFDotRbdGK0nnJoA=w74-h74-p-k-no-nu)
![A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is : (1) Degenerate primer sequence (2) Okazaki sequences (3) Palindromic Nucleotide sequences (4) Poly(A) tail sequences [NEET 2021]](https://blogger.googleusercontent.com/img/a/AVvXsEi2a2OBRDvCMkaT5C5EybwBEb296XWnq1X5tK2tI-fXN3Fd9TK60CCIfa_iLGeCLaZ_0KWdMvpYQTFkj0S8e5L1-z6kmNCFZc5yoxRtlcoC8uD1RlcJ94TiuN6Fr6mN_DYCC7XUYD3eAxb47alZpNpE0GJCN5ZGuj34H9Hzz87065dJvN22oBP9wpxC2Q=w74-h74-p-k-no-nu)
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
Calcium : Case study from NCERT for NEET
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
To transform a bacterial cell with recombinant DNA, which of the following is required? (1) 90°C temperature (2) Divalent calcium ions (3) Selectable marker (4) Restriction enzyme digested DNA
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
During the process of gel electrophoresis, DNA moves towards the _ A_ of the electrophoretic chamber as the charge of DNA is _ B_. Choose the option that fills the blanks correctly.
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
DNA fragments separated by gel electrophoresis are shown. Mark the correct statement :
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
The first recombinant DNA was constructed by linking an antibiotic resistant gene with the native plasmid of 1. Escherichia coli 2. Salmonella typhimurium 3. Clostridium butylicum 4. Acetobacter aceti
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
Downstream processing does not involve 1. Biosynthesis 2. Separation 3. Purification 4. Centrifugation
Class 12 | Biology | chapter 11 | Biotechnology: Principles and Processes
The figure below shows three steps (A, B, C) of polymersase chain reaction (PCR). Select the correct identification together with what it represents
![Antimicrobials [ NEET PYQs Topic: case study from All subject NCERT]](https://blogger.googleusercontent.com/img/a/AVvXsEitw3PPmUzps2W5lEGFejRF4C5wGr37S-YwvZLRFB8QK9AlzxazHuzcj3fLjUvxdchfayo42fQ6n7oQNpu8w3mvSTLlV6ZX2bt07qs6f9xgdLscikNLMojvC3W3RWkixG8zxU0VmZHsk_CBx20TvsselV5a14e9kKlxlUCPS3oZaj5mF112TPj9ncRATQ=w74-h74-p-k-no-nu)
Class 12 | Biology | chapter 12 | Biotechnology and its Applications
The genetically-modified (GM) brinjal in India has been developed for (a) insect-resistance (b) enhancing shelf life (c) enhancing mineral content (d) drought-resistance
Class 12 | Biology | chapter 12 | Biotechnology and its Applications
Which of the following is a wheat variety rich in proteins and developed through biofortification? (1) Himgiri (2) Atlas 66 (3) Sonalika (4) Kalyan Sona
