Q. Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β β-galactoside production and the recombinant plasmid is inserted in an E.coli strain, 1. it will lead to the lysis of host cells. 2. it will be able to produce a novel protein with dual abilities. 3. it will not be able to confer ampicillin resistance to the host cell. 4. the transformed cells will have the ability to resist ampicillin as well as produce β β-galactoside. [NEET 2021]

 Q.
Plasmid pBR322 has a PstI restriction enzyme site within gene
 

ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$$\beta$-galactoside production and the recombinant plasmid is inserted in an E.coli strain,
1. it will lead to the lysis of host cells.
2. it will be able to produce a novel protein with dual abilities. 
3. it will not be able to confer ampicillin resistance to the host cell. 
4. the transformed cells will have the ability to resist ampicillin as well as produce $\beta$$\beta$-galactoside.

[NEET 2021]

Ans: 3.

It will not be able to confer ampicillin resistance to the host cell.

Insertional inactivation: Insertion of GOl within antibiotic resistance gene/selectable marker results in inactivation / formation of the coded product.

Topic : Cloning Vectors