FACT/DEFINITION TYPE QUESTIONS
Question.
The experiment material used by Van Neil, to prove that
O2
comes out from water was studied on
(a) Chlorella pyrenoidosa
(b) Cladophora
(c) purple & green sulphur bacteria
(d) blue green algae
Answer
(c) By studying purple sulphur bacteria and green sulphur bacteria, Cornelius Van Niel was the first scientist to demonstrate that photosynthesis is a light-dependent redox reaction in 1931, in which hydrogen from an oxidizable compound reduces carbon dioxide to cellular materials. This can be expressed as: 2 H2A + CO2 ® 2A + CH2O + H2O, where A is the electron acceptor. His discovery predicted that H2O is the hydrogen donor in green plant photosynthesis and is oxidized to O2.
Video refrence -Van Neil's experiment and his contribution in photosynthesis. - YouTube
Question.
How can we separate leaf pigments of any green plant?
(a) Column chromatography
(b) Paper chromatography
(c) Electrophoresis
(d) Radio-isotopes
Answer
(b) Paper chromatography is an analytical method that is used to separate coloured chemicals or substances, especially pigments. This can also be used in secondary or primary colours in ink experiments. Leaf pigments of any green plants can be separated by using paper chromatography. A chromatographic separation of the leaf pigments shows that the colour we see in leaves is due to a single pigment but due to four pigments: chlorophyll a (bright or blue green in the chromatogram), chlorophyll b (yellow green), xanthophylls (yellow) and carotenoids (yellow to yellow orange).
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